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植物样品取样及样的预处理之办法

1 取样

准备好以下材料:

  • 1.5ml离心管,3mm钢珠
  • 镊子
  • 滤纸
  • 记号笔
  • 液氮

首先,将各样品塞入1.5ml离心管内,并加入钢珠2颗,写上标签。放入液氮。

2 预处理

  1. 将所有样品,放入板夹内,球磨仪内粉碎。
  2. 球磨仪打碎样品后,镊子取出钢珠。
  3. 按照试剂盒进行处理。

文献整理规则

全英文键入名称

范式 : METHODS-220730-[Na+/K+]-A rapid quantification method for tissue Na+ and K+ concentrations in salt-tolerant and susceptible accessions in Vigna vexillata (L.) A. Rich.

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METHODS

GROUPMEETING

ARTICLE-正在写作的文章

STUDY-正在研究的内容

可选第三词条:

研究内容所涉及基因

研究内容所涉及指标

研究内容所涉及方法

STUDY-220730-[M.ALBUS&salt]-7个草木樨品种的耐盐性研究

METHODS-220730-[细胞融合]-紫花苜蓿和白花草木樨细胞融合技术研究_贺辉

ARTICLE-220730-[RAB&function]-Rab家族及其在囊泡运输中的作用_管睿

ARTICLE-220730-[&lncRNA&mRNA]-Significance of miRNA in enhancement of flavonoid biosynthesis

ARTICLE-220730-[miRNA]-Significance of miRNA in enhancement of flavonoid biosynthesis

STUDY-220730-[WD40&stress]-rticle Genome Wide Identification and Characterization of Apple WD40 Proteins and Expression Analysis in Response to ABA, Drought, and Low Temperature

ARTICLE-220730-[MYB-bHLH-WD40]-Identification and characterization of MYB-bHLH-WD40 regulatory complexes controlling proanthocyanidin biosynthesis in strawberry (Fragaria 9 ananassa) fruits

STUDY-220730-[bHLH&salt]-Tartary Buckwheat R2R3-MYB Gene FtMYB3 Negatively Regulates Anthocyanin and Proanthocyanin Biosynthesis

STUDY-220730-[MYB]-Tartary Buckwheat R2R3-MYB Gene FtMYB3 Negatively Regulates Anthocyanin and Proanthocyanin Biosynthesis

STUDY-220730-[Salt&综述]-Physiological roles of nonselective cation channels in plants: from salt stress to signalling and development

STUDY-220730-[SALT&miRNA]-紫花苜蓿与蒺藜苜蓿盐胁迫应激调控蛋白和 miRNA 的鉴定分析

STUDY-220730-[TTG1&WD40&salt]-Genomic analysis ofWD40 protein family in the mango reveals aTTG1 protein enhances root growth and abiotic tolerance in Arabidopsis

STUDY-220730-[Salt&M.ALBUS&生理]-The Effect of Sowing Density and Different Harvesting Stages on Yield and Some Forage Quality Characters of the White Sweet Clover (Melilotus albus)

GROUPMEETING-220730-[HIS1-3 WRKY1&SALT]-Linker histone variant HIS1-3 and WRKY1 oppositely regulate salt stress tolerance in Arabidopsis

ARTICLE-220803-[RAB&function]-Protein family review The Rab GTPase family

ARTICLE-220803-[RAB&evolution]-The Arabidopsis Rab GTPase family: another enigma variation

ARTICLE-220803-[RAB&function]-Rab GTPases in vesicular transport Introduction Marino Zerial and Harald Stenmark

ARTICLE-220803-[RAB&structure]-The Mammalian Rab Family of Small GTPases: Definition of Family and Subfamily Sequence Motifs Suggests a Mechanism for Functional Specificity in the Ras Superfamily

仓央嘉措

​ 仓央嘉措(六世达赖喇嘛,民歌诗人)一般指第六世达赖喇嘛·仓央嘉措

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qrt-pcr实验流程

1 设计引物

  • 使用perlprimer 5软件
  1. 导入需要实验的基因的cds(即mrna)列,gene序列
  2. 设置退火温度范围,碱基数范围
  3. 交公司合成
  • 或另一种办法,使用NCBI pcr引物设计工具 ,参数调为150-200 设计即可
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